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Immunoblot Tests

Allergy Testing Products

Immunoblot Tests (Western blot)

Product Number (Panel 1): WB-AGE-001
Product Number (Panel 2): WB-AGE-002
Product Number (Panel 3): WB-AGE-003
Product Number (Panel 4): WB-AGE-004

Product Description

Biopanda Immunoblot Allergen Test kits test for a wide range of allergens in human serum samples. These products are used to detect the level of human specific IgE in serum, assisting health professionals in determining specific allergens affecting patients.

Immunoblot tests - Allergy Testing

Allergens to be tested include:

  • Pollen
  • Dust Mites
  • Mould
  • Animal Dander and Cockroaches
  • Food
Immunoblot tests - Allergy Testing

Principle of the assay

The allergen protein is bound to the surface of nitrocellulose membranes. When serum containing allergen-specific IgE antibodies is added to the nitrocellulose membrane, the IgE antibodies bind to the allergen protein forming an antibody-allergen immune complex. Next, Biotin-anti-human IgE antibody complex is added which binds to this antibody-allergen complex. Following the addition of an Alkaline phosphatase (AP)-streptavidin conjugate, a chromogenic reaction occurs upon contact with the substrate which is added in the final step. The colour intensity of the line/point representing the concentration of the specific allergen antibody in the serum can then be identified by a colour chart or scanner.

Four panel tests are available:

Panel 1

PositionInitialTest Item(s)
0-1 Control
1 Tx4 Mixed Tree pollens (Oak, Elm, Sycamore, Willow, Cottonwood)
2 Mx1 Mixed Moulds (Penicillium chrysogenum, Alternaria alternate, Aspergillus fumigatus, Cladosporium herbarum)
3 T70 Mulberry
4 W1 Common Ragweed
5 W6 Mugwort
6 E2 Dog dander
7 E1 Cat dander
8 I6 Cockroach
9 H1 House dust
10 D1 (Dust Mites) Dermatophagoides pteronyssinus

Panel 2

PositionInitialTest Item(s)
0-1 Control
1 F91 Mango
2 F210 Pineapple
3 F202 Cashew nut
4 F23 Crab
5 F24 Shrimp
6 F3 Codfish
7 F88 Mutton
8 F27 Beef
9 F1 Egg white
10 F2 Milk

Panel 3

PositionInitialTest Item(s)PositionInitialTest Item(s)
0-1 Control 0-2 Control
1 Tx4 Mixed Tree pollens (Oak, Elm, Sycamore, Willow, Cottonwood) 11 F210 Pineapple
2 Mx1 Mixed Moulds (Penicillium chrysogenum, Alternaria alternate, Aspergillus fumigatus, Cladosporium herbarum) 12 F202 Cashew nut
3 T70 Mulberry 13 F91 Mango
4 W1 Common Ragweed 14 F23 Crab
5 W6 Mugwort 15 F37 Common mussel
6 E2 Dog dander 16 F27 Beef
7 E1 Cat dander 17 F24 Shrimp
8 I6 Cockroach 18 F2 Milk
9 H1 House dust 19 F1 Egg white
10 D1 (Dust Mites) Dermatophagoides pteronyssinus

Panel 4

PositionInitialTest Item(s)PositionInitialTest Item(s)
0-1 Control 0-2 Control
1 F210 Pineapple 15 F91 Mango
2 w14 Common pigweed 16 F27 Beef
3 Tx4 Mixed Tree pollens (Oak, Elm, Sycamore, Willow, Cottonwood) 17F88 Mutton
4 Mx1 Mixed Moulds (Penicillium chrysogenum, Alternaria alternate, Aspergillus fumigatus, Cladosporium herbarum) 18 F37 Common mussel
5 Gx1 Grass pollens mixes (Kentucky blue, Meadow fescue, Orchard, Redtop, Rye-grass, Timothy grass, Sweet vernal grass) 19F3 Codfish
6 T70 Mulberry 20 F23 Crab
7 W22 Japanese Hop 21 F24 Shrimp
8 I6 Cockroach 22 F41 Salmon
9 E2 Dog dander 23 F80/F338 Lobster/Scallop
10 E1 Cat dander 24 F14 Soybean
11 H1 House dust 25 F13 Peanut
12 D1/D2 (Dust Mites) Dermatophagoides pteronyssinus/ Dermatophagoides farinae 26 F202 Cashew nut
13 W6 Mugwort 27 F2 Milk
14 W1 Common Ragweed 28 F1 Egg white

Storage and expiry date

These kits are stable for 24 months at 2-8°C when stored in a dark and dry environment.


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