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ELISA Test Kits

Fertility


Testosterone ELISA Kit


Product Number: ELISA-TTS-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Testosterone ELISA test kit provides a means for the quantitative determination of Testosterone in human serum or plasma.

Principle of the test:

The testosterone ELISA is based on the principle of competitive binding between testosterone in the test specimen and testosterone-HRP conjugate for a constant amount of rabbit anti-testosterone. Goat anti-rabbit IgG-coated wells are incubated with the samples, testosterone-HRP Conjugate and rabbit anti-testosterone antibody at 37oC. During the incubation, the fixed amount of testosterone-HRP conjugate competes with the endogenous testosterone in the sample for binding to the rabbit anti-testosterone antibody. Thus, if there is a high concentration of testosterone in the sample, then there will be a significant reduction in signal output of the assay as the amount of testosterone-HRP conjugate bound to the rabbit anti-testosterone antibody in the well decreases. Conversely, if there is little testosterone in the sample, there will be minimal reduction in signal. Unbound testosterone- HRP conjugate is then removed. A blue colour is formed when substrate TMB is added, which can be read spectrophotometrically at 450 nm. The intensity of the colour formed is proportional to the amount of enzyme present and is inversely proportional to the amount of unlabelled testosterone in the sample. A standard curve of absorbance against testosterone standard concentrations is plotted and the quantity of testosterone in the unknown sample is read off from the graph.

Materials and Components:

  • Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells
  • Testosterone Reference Standards: 0, 0.1, 0.5, 2.0, 6.0 and 18.0 ng/ml. Liquids, 0.5 mL each, ready to use.
  • Rabbit Anti-Testosterone Reagent, 7 mL
  • Testosterone-HRP Conjugate Reagent, 12 mL
  • TMB Substrate, 12 mL.
  • Stop Solution, 12mL.
  • Wash Buffer Concentrate(50X), 15mL

Storage:

Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8°C. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months


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