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Aflatoxin M1 ELISA Kit


Product Number: ELISA-AFM1-001

Pack Size

1 x 96 well Plate

Product Description

The Biopanda Aflatoxin M1 ELISA quantitatively detects the presence of Aflatoxin M1 in dairy products and milk powder. Aflatoxin M1 is the principal hydroxylated aflatoxin metabolite present in the milk of dairy cows fed a diet contaminated with Aflatoxin B1. Aflatoxins are carcinogenic and are highly implicated in hepatocellular carcinoma in humans.

SampleDetection LimitRecovery Rate
Milk
0.05 ppb
70-110%
Milk Powder
0.05 ppb
70-110%
Yoghurt
0.1 ppb
50-100%
Cheese
0.05 ppb
70-100%

Principle

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of Aflatoxin M1. An unknown amount of Aflatoxin M1 present in the sample and the fixed amount of Aflatoxin M1 antigens pre-coated on the wells of microtiter plate/strips compete for the anti-Aflatoxin M1 antibodies, which in turn is detected with  enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution.  The absorbance value of the developed colour is inversely proportional to the amount of the Aflatoxin M1 in the sample. The quantity of Aflatoxin M1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.   

Kit Components

  • 1 x Pre-coated Microtiter plate (12 x 8 microwells)
  • 1 x Aflatoxin M1 Enzyme conjugate (6 ml)
  • 1 x Aflatoxin M1 Antibody solution (6 ml)
  • 1 x Wash buffer concentrate (10×, 50 ml)
  • 1 x Sample Diluent concentrate (10×, 20 ml)
  • 6 x Aflatoxin M1 standards (1.0 ml/each) (0 ng/ml, 0.05 ng/ml, 0.15 ng/ml, 0.3 ng/ml, 0.6 ng/ml, 1.8 ng/ml)
  • 1 x TMB solution (12 ml)
  • 1 x Stop solution (14 ml)
  • 1 x Package insert
  • 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months


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