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Oxytetracycline ELISA Kit


Product Number: ELISA-OTC-001

Pack Size

1 x 96 well Plate

Product Description

The Biopanda Oxytetracycline ELISA kit is an indirect competitive assay to quantitatively detect the presence of Oxytetracycline in seafood and meat samples. Oxytetracycline (OTC) is a broad-spectrum antibiotic, active against a wide variety of bacteria.

SampleDetection LimitRecovery Rate
Seafood
6.0 ppb
75-105%
Meat
6.0 ppb
75-105%

Principle

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of Oxytetracycline residue. An unknown amount of Oxytetracycline present in the sample and the fixed amount of Oxytetracycline antigens pre-coated on the wells of microtiter plate/strips compete for the anti-Oxytetracycline antibodies, which in turn is detected with enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The absorbance value of the developed colour is inversely proportional to the amount of the Oxytetracycline in the sample. The quantity of Oxytetracycline in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.   

Kit Components

  • 1 x Pre-coated Microtiter plate (12 x 8 microwells)
  • 1 x Oxytetracycline Enzyme conjugate (7 ml)
  • 1 x Oxytetracycline Antibody solution (7 ml)
  • 1 x Wash buffer concentrate (20×, 30 ml)
  • 5 x Oxytetracycline standards (10×, 0.5 ml/each) (0 ppb, 4 ppb, 12 ppb, 36 ppb, 108 ppb)
  • 1 x Oxytetracycline high concentrate standard (0.5 ml)
  • 1 x TMB solution (12 ml)
  • 1 x Assay buffer concentrate (20×, 10 ml)
  • 1 x Stop solution (12 ml)
  • 1 x Package insert
  • 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months


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