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SEM ELISA Kit


Product Number: ELISA-SEM-001

Pack Size

1 x 96 well Plate

Product Description

The Biopanda SEM ELISA kit quantitatively detects the presence of SEM in meat, seafood, and honey samples. Nitrofurazone (SEM) is one of the nitrofurans, which are synthetic broad-spectrum antibiotics. Nitrofurans have been banned on treating animals used for food production, because of the possibility of increased cancer risk if people are exposed to them over a long period of time.  

SampleDetection LimitRecovery Rate
Meat/Seafood
0.1 ppb
90±10%
Honey
0.1 ppb
85±15%

Principle

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of SEM. An unknown amount of SEM present in the sample and the fixed amount of SEM antigens pre-coated on the wells of microtiter plate/strips compete for the anti-SEM antibodies, which in turn is detected with  enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution.  The absorbance value of the developed colour is inversely proportional to the amount of the SEM in the sample. The quantity of SEM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.      

Kit Components

  • 1 x Pre-coated microtiter plate (12 x 8 microwells)
  • 1 x Enzyme conjugate solution (12 ml)
  • 1 x SEM Antibody solution (7 ml)
  • 1 x Wash buffer concentrate (20×, 40 ml)
  • 1 x Extraction solution concentrate (2×, 50 ml)
  • 6 x SEM standards (1.0 ml/each) (0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb)
  • 1 x High Concentration of the standard (1.0 ml, 100 ppb)
  • 1 x Derivative Reagent (10 ml)
  • 1 x TMB substrate solution (14 ml)
  • 1 x Stop solution (14 ml)
  • 1 x Package insert
  • 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months


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