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ZIKA Virus real time RT-PCR Test Kit


Product Number: PCR-ZIKA-001

Pack Size

25 reactions

Intended Use

PCR Product The Biopanda Zika Virus real time RT-PCR Kit is an assay for the detection of Zika Virus in human serum or plasma samples.

Introduction

The Zika virus is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime- active Aedes mosquitoes, such as A. aegyti and A albopictus. Its name come from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to dengue, yellow fever, Japanese encephailitis, and West Nile viruses.

As of February 2016, there is evidence to suggest that Zika fever in pregnant women may cause abnormal brain development in the foetus through mother to child transmission, which may result in miscarriage or microencephaly.

Kit Components

  • Reaction mix 1 x 480 µl
  • Enzyme mix 1 x 28 µl
  • Molecular grade water 1 x 400 µl
  • Internal Control 1 x 30 µl
  • Zika positive control (1 x 107copies/ml) 1 x 30 µl

Principle

The principle of the real-time detection is based on the fluorogenic 5' nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

Storage Conditions

All components must be stored at -20°C in the dark immediately after receipt. Do not use reagents after the date of expiry printed on the package. Avoid repeated thawing/freezing of the reagents. If necessary aliquot kit components.

Storage Conditions

+ 2 - 8 °C

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